Anti-detection test method using the above enzyme-linked immunosorbent (ELISA) -C-100 has the following disadvantages: 1 anti -C-100 appeared later, after about half of the blood transfusion hepatitis C patients after 4 to 6 months of anti -C -100 first seroconversion, therefore, not as a routine laboratory diagnosis of acute hepatitis C; 2 anti -C-100 is not a neutralizing antibody, nor is lgM antibodies, but lgG antibodies; 3 of this Act are not sensitive enough, a few hepatitis C patients with undetectable anti -C-100; 4. non-specific, some home autoimmune chronic liver disease can occur in patients with false-positive, therefore, the need for anti-HCV-positive recombinant immunoblot test (Recombinant Immune Blot Assay, RIBA, or called Western Blot) confirmed.
Because HCV core antibody appeared earlier, therefore, the recent U.S. second-generation enzyme-linked immunosorbent assay method (ELISA) to detect anti-HCV. The kit uses HCVC region encoding protein C-22-3 and non-structural region encoding NS3 protein C-33-3 and C-100-3 coated carrier. Law detection by anti-HCV, the detection rate can be increased from 25 to 30%, and the detection of anti-HCV of time can be 16 to 42 days in advance.
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